Test sample:	Potato
Test location:	Mr. E’s farm in Tomisato city, Chiba prefecture
Planting density:	Ridge spacing: 100cm, intrarow spacing: 30cm
Survey method:	Comparative survey between the yields of the whole area mixed with 80kg / 10a of Darwin 1000 (Darwin area) before planting in late February 2007, and the yield of traditional method.
Survey result:
5 potatoes were taken from each area, measured three times and averagedSurvey date: June 26th, 2007 Area Weight of 5 potatoes (g) Weight of each potato (g) Weight of 10a yield (kg) Darwin 6,474 156.6±62.5 4,316.0 (105.7% compared to traditional method) Traditional 6,124 151.8±67.9 4,082.9

The average weight of one potato in Darwin area was heavier than in the traditional area, and the variation in weight decreased. Darwin area has increased yield by about 6% compared to the traditional area. Scab disease seems to be less likely to occur in Darwin area compared to the traditional area (photo).

Comparison of scab disease occurrence in potatoes (Left: Darwin Right: Traditional)

Test sample:	Lettuce
Test location:	Mr. K’s farm in Minamimaki village, Nagano prefecture
Sowing date:	3/12/2007
Number of plug holes:	200 holes
Survey method:	Seedlings are cultivated in an area with 500g of Darwin 2000 mixed into 40 liters of soil, and another area using conventional method, and compared the weight and tension of roots.
Survey result:
10 stubs were taken randomly from each area Survey date: April 4th, 2007 There is increased root weight in the area with 500g of Darwin 2000 mixed into 40 liters of soil before planting (roughly 18% increase compared to the conventional area) and the root tension is also improved. Comparison of lettuce seedlings (Top: conventional, Bottom: Darwin)

Test sample:	Apple (Fuji)
Test location:	Nagano Nanshin Agricultural Experiment Station
Spraying time:	Solution with 1:1000 ratio was sprayed 5 times every 20 days immediately after leaves started falling
Survey date: November 11th, 2007 Area Bitter pit incidence (%) Crack incidence rate (%) Fruit weight (g) Sugar content (%) Acidity (%) Fruit skin color Hardness in 1bs of sun exposed surface area Surface color Soil color Darwin 0.0 24.1 345.3 15.9 0.32 3.9 5.5 13.4 Untreated 0.0 82.0 279.6 15.9 0.35 3.1 4.8 13.1
Test location:	Nagano Prefecture Fruit Experiment Station
Spraying time:	Solution with 1:1000 ratio was sprayed 5 times every 20 days immediately after flowers started falling
Survey date: November 26th, 2003 Area Number of fruits Fruits / tree Bitter pit (%) Fruit weight (g) Sugar content (%) Acidity (%) Fruit skin color Hardness in 1bs of sun exposed surface area Incidence rate (%) Incidence degree Surface color Soil color Darwin 140 2.6 1.9 361 15.5 0.32 4.8 6.2 13.0 Traditional 125 3.2 1.8 386 15.2 0.37 4.6 6.2 12.8 Unsprayed 108 4.2 2.9 368 16.0 0.37 5.2 6.5 13.5

Production area:	Hirosaki city, Aomori prefecture
Spraying time:	15 days after flowers started falling, solution with 1:1000 ratio was sprayed every 10~14 days

Production area:	Hokuto city, Yamanashi prefecture	Comparison of cultivation of grapes (Left: Sprayed with Darwin, Right: Untreated) * Some fruits have been removed to make the comparison of vines easier to understand.
Variety:	Seedless Pione
Purpose of spraying:	Flesh enrichment, prevention of shedding
Purpose of spraying:	Twice before sacking, once after sacking
(2) Peach
Production area:	Minami Alps city, Yamanashi prefecture and Fukushima city, Fukushima prefecture
Variety:	All varieties from early ripening to late ripening variety
Purpose of spraying:	Flesh and sunlight retention improvement
Spraying time:	Twice after desacking